Vascular endothelial growth factor and hypoxia-inducible factor-1α gene polymorphisms and coronary collateral formation in patients with coronary chronic total occlusions.

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Authors
Amoah, Vincent
Cotton, James Matthew
Holroyd, Eric
Smallwood, Andrew
Wrigley, Benjamin
Issue Date
2016-06-24
Journal
Type
Article
Peer-Reviewed Publication
Keywords
Cardiovascular disease
Chronic total occlusion
Gene polymorphism
Hypoxia-inducible factor 1a
Peer-reviewed article
Polymerase chain reaction
Rentrop grade
Vascular endothelial growth factor
Journal Title
SAGE Open Medicine
Volume
4
Issue
Begin page
End page
Abstract
Introduction: We evaluated the association between two single nucleotide polymorphisms of the vascular endothelial growth factor gene and one of the hypoxia-inducible factor-1α gene and the degree of coronary collateral formation in patients with a coronary chronic total occlusion. Methods: Totally, 98 patients with symptomatic coronary artery disease and a chronic total occlusion observed during coronary angiography were recruited. Genotyping of two vascular endothelial growth factor promoter single nucleotide polymorphisms (-152G>A and -165C>T) and the C1772T single nucleotide polymorphism of hypoxia-inducible factor-1α were performed using polymerase chain reaction and restriction fragment length polymorphism analysis. The presence and extent of collateral vessel filling was scored by blinded observers using the Rentrop grade. Results: We found no association between the vascular endothelial growth factor -152G>A, -165C>T and hypoxia-inducible factor-1α -1772C>T with the presence and filling of coronary collateral vessels. A history of percutaneous coronary intervention and transient ischaemic attack/cerebrovascular accident were associated with the presence of enhanced collateral vessel formation following binary logistic regression analysis. Conclusion: The study findings suggest that coronary collateral formation is not associated with the tested polymorphic variants of vascular endothelial growth factor and hypoxia-inducible factor-1α in patients with symptomatic coronary artery disease and the presence of a chronic total occlusion.
Citation
Amoah V, Wrigley B, Holroyd E, Smallwood A, Armesilla AL, Nevill A, Cotton J. Vascular endothelial growth factor and hypoxia-inducible factor-1α gene polymorphisms and coronary collateral formation in patients with coronary chronic total occlusions. SAGE Open Med. 2016 Jun 24;4:2050312116654403. doi: 10.1177/2050312116654403. PMID: 27621802; PMCID: PMC5006805.